A sensitive ultra-performance liquid chromatography-electrospray tandem mass spectrometric method（UPLC-MS/MS） combined with solid phase extraction（SPE） was established for the determination of eighteen steroid hormones，including prednisolone，prednisone，methylprednisolone，betamethasone，dexamethasone，trenbolone，prednisolone acetate，hydrocortisone acetate，nortestosterone，testosterone，stanozolol，norethindrone，hydroxyprogesterone，methyltestosterone，androstenedione，megestrol acetate，progesterone and medroxyprogesterone acetate in the influent and final effluent from wastewater treatment plant（WWTP）.The operation conditions influencing the SPE performance were optimized by single factor experiments and orthogonal test，and UPLC-MS/MS experimental parameters were also studied in detail.The results indicated that，under the optimal conditions， the calibration curves of eighteen steroid hormones were linear in the range of 1.0-200 μg?L-1 with their correlation coefficients（r2） not less than 0.997 6.The detection limits for the method ranged from 1.49 ng?L-1 to 10.59 ng?L-1.The recoveries of WWTP water samples at spiked levels of 20-100 ng?L-1 were between 67% and 109% with RSDs not more than 13.9%.The method was applied in the determination of steroid hormones in wastewater from four sewage treatment plants in Nanjing.Nortestosterone，androstenedione，testosterone，hydroxyprogesterone，prednisone and prednisolone were found in the influent of the four sewage treatment plants，in which the concentration of prednisone was the highest.A certain amount of steroid hormones were also found in the final effluent.