Biosensors and bioanalysis based on nanomaterials are the frontlines of current bioanalytical research.Much attention has been drawn to nanoparticles in electrochemical biosensors and bioanalysis due to their advantages of enhancing the sensitivity of the biosensors.The applications of nanomaterials in electrochemical immunosensors and DNA biosensors are reviewed.The prospects of the multi-labed nanoparticals,which have been used for biomolecular recognition and enhancing signal,are discussed emphatically for the simultaneous multianalyte detection in bioanalysis.