An electrochemical sensing platform was developed based on the composite of ionic liquid（1-butyl-3-methylimidazolium dicyanamide,［BMIM］N（CN）2，IL）,multi-walled carbon nanotubes（MWCNTs） and polymeric matrix（chitosan,CS）.Scanning electron microscope images showed that MWCNTs had a good dispersibility in the MWCNTs-IL-CS composite and formed a relative smooth film with unique structure.Electrochemical studies indicated that the MWCNTs-IL-CS composite provided a synergistic improvement on the electrochemical oxidation of dopamine（DA）,uric acid（UA） and ascorbic acid（AA） by the decrease of their anodic over-potentials and the enhancement of their amperometric responses.The peak separations between UA and DA,DA and AA were 114 mV and 208 mV,respectively.Additionally,the MWCNTs-IL-CS composite modified glassy carbon electrode（MWCNTs-IL-CS/GCE） showed a good analytical performance in the simultaneous determination of DA,AA and UA.The calibration curves of 5.00-425 μmol/L,0.020-2.25 mmol/L and 0.050-10.0 mmol/L,and the detection limits（S/N=3） of 1.01,10.7 and 42.9 μmol/L were obtained for DA,AA and UA,respectively.With good selectivity and sensitivity,the present method is promising in the simultaneous determination of DA,AA and UA in real samples.