An ultra performance liquid chromatography-electrospray ionization triple-quadruple tandem mass spectrometric（UPLC-ESI MS/MS) method was established for the determination of residues of carbadox（CBX)，quinocetone（QCT)，mequindox（MEQ)，quinoxaline-2-carboxylic acid（QCA) and 3-methylquinoxaline-2-carboxylic acid（MQCA) in aquatic products.The samples were separately extracted with acetonitrile-ethyl acetate（50∶50) mixed solution and phosphate buffer，then purified with n-hexane.The separation of CBX，QCT，MEQ，QCA and MQCA was carried out on an ACQUITYTM BEH C18 column（1.7 μm，2.1 mm i.d.×100 mm) using methanol-acetronitril（11∶3) and 0.1% formic acid as mobile phases.The target compounds were confirmed and quantified by MS-MS under multiple-reaction monitering（MRM) mode with the external standard method.The results showed that there were good linear relationships between peak area and concentrations of five quinoxalines in the range of 5-50 μg/L，with correlation coefficients more than 0.998 and limits of quantitation（LOQs) of 1.0 μg/kg.The recoveries at spiked levels of 1.0-10 μg/kg ranged from 70% to 108% with relative standard deviations of 2.5%-11%.This method shortened the time of pre-treatment process，and the result was credible.It could satisfy the requirement of mass and fast analysis in the laboratory.