A new ultrasonic-assisted extraction followed by dispersive liquid-liquid micro-extraction（DLLME） and GC-MS analysis method was developed for the simultaneous determination of eight alkenylbenzenes and other flavor-related compounds including safrole,coumarin,6-methylcoumarin,7-metheoxycoumarin,estragole,methyleugenol,pulegone and thujone in tobacco additives.The different parameters affecting the whole process such as the type and volume of extraction and disperser solvents,the salt addition and the extraction time were studied.Under the optimum conditions,the enrichment factors（EF） ranged from 140 to 208.The linear relationships of eight alkenylbenzenes and other flavor-related compounds were obtained in the range of 0.4-928 μg/L,with correlation coefficients（r2） over 0.998 9.The limits of detection（LOD） and the limits of quantitation（LOQ） were in the ranges of 0.04-0.24 μg/L and 0.13-0.80 μg/L,respectively.The average recoveries were in the range of 90%-100% with relative standard deviations（RSDs） of 2.4%-6.7%.The method was successfully applied in the analysis of real tobacco flavor additive samples.Compared with the industry recommended standard method,lower concentrations of detection were achieved due to the high enrichment power of DLLME.Therefore,this method shows tremendous potential in trace analysis of alkenylbenzenes and other flavor-related compounds in real samples.