A nickel hydroxide-multi-wall carbon nanotubes modified ceramic carbon electrode(Ni(OH)2/MWNT/CCE) was fabricated by using surface coating method and cyclic voltammetry，and characterized by XRD technique and scanning electron microscopy(SEM).The electrocatalytic activity for glucose on the modified electrode was investigated by cyclic voltammetry and amperometric method.The results showed that the modified electrode exhibited a high electrocatalytic activity for the oxidation of glucose.Based on this，an amperometry technique for the determation of glucose was established.Under the optimum conditions，the oxidation peak current had linear relationships with concertration of glucose over the ranges of 2.0×10-7-5.7×10-4 mol?L-1(r=0.999 9 and 5.7×10-4-2.7×10-3 mol?L-1 (r=0.999 1)，with sensitivities of 2 786.5 μA?(mmol?L-1)-1?cm-2and 2 005.2 μA?(mmol?L-1)-1?cm-2，respectively.The detection limit (3sb) was 8.0×10-8 mol ? L-1.The proposed method was successfully applied in the detection of glucose in serum.