A fingerprint analysis method was developed for the quality control of Wanglaoji Guangdong herbal tea granule(GHTG)by ultra high performance liquid chromatography(UHPLC).The samples were extracted for 30 min by ultrasonic extraction using methanol as solvent.UHPLC separation was performed on a Waters ACQUITY HSS T3 C18(3.0 mm× 150 mm,1.8 μm) column by gradient elution with 0.5% formic acid-acetonitrile as mobile phase,at the temperature of 35 ℃ and a flow rate of 0.8 mL/min.A good separation of 28 common peaks was achieved within 18 min,and 4 of them were confirmed by reference substances.Similarity evaluation was performed by a professional software named Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004A),and the similarity among 23 batches of samples was no less than 0.95.The principal component analysis(PCA)also showed that the 23 batches of samples were located in one area.The results of similarity evaluation and PCA confirmed that the quality of Wanglaoji GHTG was stable,and the developed fingerprint analysis was fast,efficient and robust for the quality control of Wanglaoji GHTG.
关键词
超高效液相色谱指纹图谱相似度评价主成分分析广东凉茶颗粒质量控制
Keywords
ultra high performance liquid chromatographyfingerprintsimilarity evaluationprincipal component analysisGuangdong herbal tea granulequality control