In alkaline conditions,4-（ 3-acryloxy-2-hydroxy ） propyloxy-β-cyclodextrin（ PCD ） was synthesized based on the reaction of 1-alklyloxy-2,3-epoxy propane（also named as allyl glycidyl ether,AGE） and β-cyclodextrin（β-CD）.A new type β-cyclodextrin polymer chiral monolithic column for CEC was prepared by using the in-situ polymerization of PCD and GMA as functional monomers in the capillary tube.The prepared monolithic column was evaluated by separating racemic mixtures of three amino acids and a chial drug（guguaifenesin） in capillary electrochromatography mode.The influences of organic modifier，buffer pH value and temperature on separation were investigated.Under the optimal CEC conditions,all of enantiomers were baseline separated in 8 min.Results showed that the novel PCD monolithic column had good chiral recognition ability,better reproducibility and easy operation.