The development and applications of ambient ionization mass spectrometry(AI-MS) that realized ionization under ambient conditions without sample pretreatment have become a frontier field in mass spectrometry and deserved much attention over the last few years.This review summarizes the basic principle and traits，application progress of ambient ionization mass spectrometry.Moreover，it introduces the fundamental characteristics and representative applications of gas flow assisted ambient ionization mass spectrometric technologies involved the author′s independent-developed air flow assisted ionization(AFAI).The future and trends of ambient ionization mass spectrometry are also discussed.