A new method for the determination of 3 aquaculture disinfectants in aquatic products using hydrophilic interaction liquid chromatography-tandem mass spectrometry（LC-MS/MS） was established.The homogenized sample was extracted with acetonitrile-5% ammonia hydroxide（30∶70，by volume）.Separation of analytes was performed using a SeQuant ZIC-HILIC hydrophilic interaction column by gradient elution after solid-phase extraction（SPE） purification.The qualitative and quantitative analyses of 3 aquaculture disinfectants were operated by electrospray ionization mass spectrometry under the negative mode using multiple reaction monitoring（MRM）.Acetonitrile and water with 0.1% formic acid and 10 mmol/L ammonium acetate were used as the mobile phase.The calibration curves were linear in the range of 0.042-2.4 mg/L with correlation coefficients more than 0.99.The average recoveries at three spiked concentration levels varied from 68% to 96% with relative standard deviations（RSDs） of 5.9%-10.9%.The limit of detection（LOD，S/N≥3） and limit of quantitation（LOQ，S/N≥10） were 0.015 mg/kg and 0.05 mg/kg，respectively.The real sample tests showed that the method was suitable for the determination of aquaculture disinfectants in aquatic products.