Testosterone is the principal anabolic steroid in human body，which plays a key role in promoting physiological function.Because of the extensive needs in clinical diagnosis and sport field，the determination of testosterone has received a considerable attention.In this paper，the methods for the detection of testosterone were summarized，including conventional instrument analysis，immunological detection and electrochemical detection，and the progress in electrochemical detection of testosterone，especially in the construction of electrochemical immunosensors were reviewed.