A high performance liquid chromatography-electrospray ionization tandem mass spectrometric（HPLC-ESI MS/MS） method was developed for the determination of seven plant growth regulator residues，including parachlorophenoxyacetic acid，gibberellic acid（GA3），2,4-dichlorophenoxyacetic acid，α-naphthaleneacetic acid，indolebutyric acid，6-benzylaminopurine and forchlorfenuron in fruits and vegetables. Plant growth regulators were extracted twice from samples with methanol and the extracts were cleaned up on a Waters C18 solid phase extraction column（SPE，0.5 g，3 mL）.The qualitation of the analytes was achieved under selective reaction monitoring（SRM） mode,and the quantification of the analytes was carried out by the external standard method .The chromatography separation was performed on a Hypersil GOLD aQ（150 mm×2.1 mm，3 μm） column by gradient elution with methanol and water as mobile phase.Under the optimal conditions，good linearities were obtained for seven plant growth regulators with their correlation coefficients no less than 0993 5.The average recoveries of seven plant regulators in fruits and vegetables samples at three spiked levels ranged from 79% to 97%，with relative standard deviations（RSDs） no more than 7.6%.The limits of detection（LODs） were in the range of 0.40-200 μg/kg.The result showed that the method was simple and sensitive，and was applicable for the detection of plant growth regulators.