To investigate the role of iASPP in human ovarian cancer progression,the expression levels of iASPP in 56 pairs of ovarian cancer tissues and the matched tissues were examined using quantitative real-time PCR.Receiver operating characteristic(ROC) analysis was performed to further evaluate the clinical value of iASPP mRNA expression for the prediction of ovarian cancer.The iASPP gene expression could be firstly silenced by an iASPP-siRNA interference method in vitro.Then,the biological impact was investigated by Hoechst 33342 and CCK-8 assay in ovarian cell line OVCA420.Comparing with the adjacent tissues,it showed an over expressed iASPP in human ovarian cancer tissues.Down regulation of siRNA mediated by iASPP resulted in inhibited proliferation and increased apoptosis of ovarian cancer cell line OVCA420.According to the results,iASPP,which showed over-expression in the progression of ovarian cancer,could be considered as a target for ovarian cancer therapy.Moreover,iASPP mRNA could be a candidate marker for the diagnosis of ovarian cancer.