An anodic stripping voltammetric method for the determination of arsenite using Au-Pd bimetallic nanoparticles（Au-Pd NPs） modified glassy carbon electrode（Au-Pd/GCE） was developed.The structural information and electrochemical activities of the synthesized Au-Pd nanoparticles were investigated with UV-Vis spectroscopy，high resolution transmission electron microscopy（HRTEM） and cyclic voltammetric（CV） method.The determination of arsenic was conducted by square wave voltammetric（SWV） method.Effects of deposition potential and SWV parameters (such as frequency，increment and amplitude) on the current intensity of arsenite were investigated.The results showed that Au-Pd bimetallic nanoparticles presented a core-shell structure in shape and the modified electrode exhibited the characteristic peaks of both Au and Pd.A sensitive anodic stripping peak of arsenite appeared at about 0.30 V，and the peak current was linear with concentration of arsenite in the range of 0.5-20 μg/L with a limit of detection（LOD） of 0.15 μg/L，which is far below the maximum guideline value（10 μg/L） set by World Health Organization（WHO）.The modified electrode exhibited a good repeatability toward the consecutive determination of arsenite.The interference experiments also showed that except for Cu（Ⅱ），the existance of Pb（Ⅱ），Cd（Ⅱ） and Zn（Ⅱ） would not affect the detection of As（Ⅲ）.