A high-performance liquid chromatography（HPLC） method for the determination of glucosamine in glucosamine sulfate potassium chloride capsules was developed.The separation of analyte was performed on a Hedera NH2 column （250 mm ×4.6 mm,5 μm） with acetonitrile and phosphate buffer （65∶35） as mobile phase,at a flow rate of 1.0 mL/min.The variable wavelength detector（VWD） was set at 195 nm.In the above chromatographic conditions,the calibration curve was linear over glucosamine concentration in the range of 1.6-2.4 g/L.The limit of detection（LOD） of glucosamine was 7.81 mg/L. The RSD（n=6） for the accuracy was 0.53% and the average recovery was 100%with RSD of 1.1%.This method is simple,accurate and with no need of derivation,and is suitable for the determination of glucosamine.