Graphene is an atomically thin honeycomb lattice of carbon atoms.It comprises a single layer of SP2-hybridized carbon atoms arrange in six-membered rings.As a new carbon-based nanomaterial after carbon nanotube，graphene could be considered as one of the ideal absorption materials with superior chemical stability，thermal stability and high specific surface area.Graphene oxide(GO) possesses rich oxygen-contained groups on its surface.Most of the graphene used nowadays is produced from the reduction of graphene oxide.In recent years，GO and graphene have been applied in the separation and enrichment of organic compounds，mental ions and biological molecules.In this paper，the preparation methods of GO and graphene and their applications in sample pretreatment techniques such as solid-phase extraction(SPE)，solid-phase microextration(SPME)，magnetic solid-phase extraction(MSPE)，stir bar sorptive extraction(SBSE) were reviewed.