A new approach was established for the simultaneous determination of eight classes of limited using veterinary drug residues,including quinolones,macrolides,sulfoamides,trimethoprim,lincosamides,nitroimidazoles,quinoxalines and peptides,in aquatic products by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）.The analytes were extracted with 1% acetic acid in acetonitrile,then purified with refrigerated centrifuge and hexane.The sample matrix-matched calibration was used to determine the residue contents by the external standard.Electrospray ionization mass spectrometry was operated in the positive mode using multiple reaction monitoring（MRM）for the qualitative and quantitative analyses of 38 analytes.The results indicated that correlation coefficients of the calibration curves were more than 0.99.The average recoveries of 38 analytes at four spiked concentration levels ranged from 43% to 123%.The intra-day relative standard deviations（RSDs） were in the range of 1.0%-26.4%,and the inter-day RSDs were 1.6%-28.9%.The limits of quantitation（LOQs,S/N≥10） were in the range of 5-20 μg/kg.The method is simple,rapid and reliable,and is suitable for the simultaneous determination of multiresidues of veterinary drugs in aquatic products.