A PABSA/GN/GCE composite-modified electrode was fabricated by using a graphene nanosheets（GN） modified glassy carbon electrode（GCE）electropolymerized with p-aminobenzene sulfonic acid（PABSA）.The electrochemical behaviors of uric acid（UA） and ascorbic acid（AA） on the nanocomposites-modified electrode were investigated.Compared with PABSA/GCE and GN/GCE,the PABSA/GN/GCE improved the resolution and the sensitivity for the determination of UA in the presence of AA.In 0.1 mol/L phosphate buffer solution（pH 7.0）,a peak to peak separation of 344 mV was observed,which enabled the selective determination of UA.The peak currents obtained from DPV were linearly related to concentrations of UA in the range of 1.0×10-7-8.0×10-4 mol/L.The detection limit was 4.5×10-8 mol/L.The nanocomposites-modified electrode was adopted for the detection of UA in the human urine with satisfactory result.