Propranolol(PPL) is a nonselective blocking agent of β-adrenoceptor and it has many pharmacology effects.Propranolol is used clinically as racemics with partes aequales of S- and R-propranolol,but the β-adrenoceptor blocking effects of S is 100 times more than that of R-propranolol，and the R form is known to have antioxidant activities.It is important in chiral separation for propranolol enantiomers pharmacokinetics.In this paper,the applications of chromatographic method in resolution of propranolol enantiomers and their pharmacokinetics were reviewed by consulting and summarizing references in order to supply a reference for their further exploitation.