A method for the determination of nine life elements（K,Ca,Ti,Mn,Fe,Ni,Cu,Zn and Rb） in pine pollen was developed by microwave digestion and total reflection X-ray fluorescence（TXRF）.The pollen was digested by microwave digestion.The counts,QXAS software and internal standard method were used for TXRF measurement.Effects of three sample preparation methods including dry ashing method，wet nitration method and microwave digestion method,on the TXRF results of certified reference material（GBW10026） were compared.The microwave digestion method was selected as the pretreatment method.Nine elements in GBW10026 were determined by TXRF with microwave digestion.The lower limit of detection（LLD） and the limit of determination （LDM） for different elements in pollen were calculated to be 0.002-0.054 mg/L and 0.004-0.122 mg/kg,respectively.The relative standard deviations（RSDs） were lower than 5.5%.This method showed advantages of easy operation,less sample consumption and low detection limit.The method was used to analyse the content of 9 elements in pine pollen,and there were was no significant difference between the results and those of ICP-MS.