A method for the analysis of 32 industrial dyes in seasoning paste was developed by ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） combined with gel permeation chromatography（GPC） purification.The homogenized sample was extracted with acetonitrile，and then purified by GPC.The targeted compounds were detected by MS/MS system with eletrospray ionization（ESI） under multiple reaction monitoring（MRM） mode.The result indicated that the limits of quantitation（LOQs） of 32 dyes were in the range of 0.2-5 μg/kg.The linear correlation coefficients of 32 dyes were more than 0.994.The average recoveries ranged from 53% to 115% with RSDs of 3.9%-22.4%.This method was suitable for the identification and quantification of multi-component dyes in seasoning paste due to its simplicity，good purification and high sensitivity.