An HPLC method was developed for the enantioseparation of 13 flavonoids on chiral columns of Chiralpak AD-H (250 mm×4.6 mm，5 μm) and Chiralcel OZ-H(250 mm×4.6 mm，5 μm).The influences of type and percentage of alcohol modifier on the chiral recognition were examined.Effects of trifluoroacetic acid(TFA) in the mobile phase were also studied.The optimized chromatographic conditions for 13 racemic flavonoids were as follows：mobile phas：n-hexane-isopropanol (ethanol)-TFA(75∶25∶0.1),flow rate:0.5 mL?min-1,column temperature：35 ℃,UV detection wavelength:290 nm.Under the optimal conditions,13 racemic flavonoids were successfully enantioseparated at the baseline on Chiralpak AD-H and Chiralcel OZ-H chiral columns.This study illustrated the potentials of Chiralpak AD-H and Chiralcel OZ-H chiral columns in the chiral separation of flavonoids and their analogues.