Compared with bulk molecularly imprinted polymer，molecularly imprinted microspheres（MIMs） exhibit more uniform size and much better dynamic characteristics.Thus，MIMs are widely used in the applications of solid-phase extraction（SPE），chromatographic separation，biosensor and drug delivery system.The main synthesis methods of MIMs are suspension polymerization，multi-swelling polymerization（MSP） and precipitation polymerization（PP）.In this review，the research literatures on MIMs published in recent 10 years were tracked.The three synthesis methods of MIMs were summarized and commented.The advantages and disadvantages of those synthesis methods were compared.Meanwhile，the applications of MIMs in SPE were reviewed，and the prospect of synthesis and applications of MIMs was also put forward.