A method based on ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） was developed for the simultaneous determination of three industrial acid dyes，namely acid orange Ⅱ，acid orange Ⅲ and acid orange AGT，in meat products.The samples were extracted with acetonitrile（containing 5% ammonia），cleaned up and enriched with WAX solid phase extraction（SPE) cartridge，and then analyzed by UPLC-MS/MS under the multiple reaction monitoring（MRM） mode.All the analytes were successfully detected in negative ion mode by adding an ion-pair reagent of dihexylammonium acetate（DHAA） to the mobile phase in 6 min.The calibration curves were linear between 5.0 μg/L and 300 μg/L，and the correlation coefficients（r） were more than 0.999.The limits of detection（LOD，S/N=3） and the limits of quantitation（LOQ，S/N=10） were in the range of 20.0-50.0 μg/kg and 70.0-170.0 μg/kg，respectively.The spiked recoveries were in the range of 83%-89%，and relative standard deviations （RSD，n=3） were no more than 7.5%.The method was simple，effective and sensitive，and was suitable in the determination and confirmation of three industrial acid orange dyes in the meat product samples.