A method for the determination of histamine,tyramine,cadaverine,putrescine,tryptamine,spermine,spermidine and phenylethylamine in wines by liquid chromatography-quadrupole/electrostatic field orbit trap mass spectrometry was developed.The samples were diluted with deionized water,followed by dispersive solid phase extraction（DSPE） clean-up procedure with 200 mg octadecylsilyl（ODS） and 50 mg primary secondary amine（PSA）.Chromatographic separation was performed on a Phenomenex C18（100 mm×4.6 mm×2.6 μm） column with acetonitrile-0.1‰ heptafluorobutyric acid（HFBA） solution as mobile phase by gradient elution.Eight biogenic amines were analyzed in positive mode with ESI interface by quadrurpole/electrostatic field orbit trap high-resolution mass spectrometry.Accurate mass extraction for the target analytes and two-stage threshold-triggered mass improved the accuracy during the analysis.The linearities of 8 biogenic amines ranged from 0.2 mg/L to 10.0 mg/L with correlation coefficients more than 0.99.The limits of quantitation for 8 biogenic amines were all 1.0 mg/L.The average recoveries at three spiked levels（1.0,5.0 and 10.0 mg/L） were between 83.7% and 101.6% with relative standard deviations（RSDs） not more than 12.9%.The developed method was simple and sensitive,and could be applied as a screen and confirmatory method for the analysis of biogenic amines in red wines.