A method for the quantitative determination of carbamazepine in blood by solid phase extraction(SPE) combining with LC-MS/MS was developed.The carbamazepine in the blood sample was extracted with SPE and separated with Waters AtlantisTM dC18column(150 mm×3.9mm,5 μm).The detection of carbamazepine was performed using electrospray ionization source,under positive ion mode and multiple reaction monitorring mode with SKF-525A as the internal standard.The calibration curve was linear in the range of 100-6 000 μg/L for carbamazepine,with a detection limit of 0.1 μg/L.The intra-day RSD(n=6) and the inter-day RSD(n=6) were both no more than 8.6%,and the recoveries were more than 80%.With the advantages of high sensitivity,good repeatability,long-time stability and specificity,this method could be used to detect the carbamazepine in forensic and clinical analytic toxicants．