A stable integrated gold film multi-electrode array,including six gold working electrodes(1 mm×2 mm),a gold counter electrode(2 mm×13 mm) and a thick-film Ag/AgCl reference electrode,was fabricated by mask technique of polyethylene sticky film and the gold sputtering technique on FR-4 glass fiber substrate.The electrochemical characteristics of the multi-electrode array fabricated were investigated using electrochemical methods.There was a negative shift of 0.067 V obtained on the thick-film Ag/AgCl reference electrode fabricated compared with a commercial Ag/AgCl/3.0 mol/L NaCl reference electrode.After one month,the potential of reference electrode did not change obviously.The surface roughness of the working electrodes was studied by scanning electrochemical microscope(SECM).The satisfied surface roughness was obtained.The reproducibility of electrochemical areas and properties of the gold working electrodes were evaluated by the cathodic peak areas in 0.10 mol/L H2SO4 aqueous solution and the Ipa/Ipc values in 5.0 mmol/L K3Fe(CN)6 aqueous solution,and satisfactory results were obtained.This work demonstrated that the multi-electrode array fabricated can be utilized as electrode system for following electrochemical studies.Multi-electrode array can be further applied as a basis electrode in the design and fabrication of electrochemical biosensor array.