Novel magnetic molecularly imprinted polymers(M-MIPs） were prepared using Sudan Ⅰ as template by molecularly imprinting technique.The morphology,composition and magnetic properties of magnetic nanoparticles were characterized by scanning electron microscope,infrared spectroscopy and vibrating sample magnetometer.The Scatchard analysis showed that there were two kinds of binding sites between the imprinted polymers and the template.The apparent maximum number(Qmax） of the M-MIPs were 84.59 μmol/g and 210.49 μmol/g,respectively.The relative selectivity coefficient of M MIPs toward Sudan Ⅰ/Sudan Ⅱ and Sudan I/Sudan Ⅲ were 2.47 and 2.24,respectively,which indicated that the M-MIPs exhibited better adsorption properties and selectivity for Sudan Ⅰ.The M-MIPs were applied in the separation of Sudan I in the extracting solution of paprika.The eluant after treating with the M-MIPs was determined by HPLC.The detection limit was 0.50 μg/g.The recoveries of Sudan I ranged from 78% to 103% with RSDs of 2.8%-5.8%.