After cellulose was dissolved in a mixture of N,N-dimethylacetamide-LiCl-pyridine mixed solution,it was directly coated onto γ-aminopropyl silica gel,then immobilized onto silica gel using 4,4′-diphenylmethane diisocyanate and hexamethylene diisocyanate as the spacer,respectively,and finally reacted with an excess of 3,5-dimethylphenyl isocyanate.Thus,two immobilized-type chiral stationary phases（CSPs） based on cellulose 3,5-dimethylphenylcarbamate were prepared and evaluated for HPLC enantioseparation.The results showed that the obtained CSPs exhibited high chiral recognition ability and solvent durability.Many of the tested racemates could be well resolved when using eluents containing chloroform or tetrahydrofuran instead of the conventional eluent（e.g.hexane-isopropanol mixture）.In addition,interfences on the chiral recognition of CSPs due to different diisocyanates could be negligible.