In this paper,from the angle of quantitative fingerprint,the volatile constituents of Herba Houttuyniae(HH) obtained from three different origins(Boluo County of Guangdong Province,Dali City of Yunnan Province and Changsha County of Hunan Province) were investigated comparatively by gas chromatography-mass spectrometry(GC-MS) combined with HELP method,and the overlapping chromatographic peaks were analyzed.The results showed that,the differences in the volatile chemical compositions of HH from three different origins are mainly embodied in the relative contents of most compounds,including terpinen-4-ol,α-terpineol,sabinen,β-myrcene,etc.The relative contents of terpinen-4-ol in three different origins were 0.19%-0.24%,4.47%-4.93%and 0.03%-0.07%，respectively,the relative contents of α-terpineol were 0.17%-0.27%,0.22%-0.24% and 0.07%-0.09%,respectively,the relative contents of sabinen were 0.48%-0.64%,25.2%-26.8% and 0.07%-0.11%,respectively,the relative contents of β-myrcene were 33.66%-43.36%,10.11%-11.11% and 51.23%-55.39%,respectively.This study can provide some reliable foundation for volatile components of Herba Houttuyniae in regulating the pharmaceutical products and the practical application.