Simultaneous separation and purification of saturated and aromatic hydrocarbons in crude oil by using solid-phase extraction（ SPE ） cartridges of silica-cyanopropyl（ SiO2/C3-CN） is presented in this paper.The results indicate that，compared with the separation effect of silica cartridges，the silica-cyanopropyl catridge’s effect is better，and saturated and aromatics hydrocarbons could be separated completely with 4.5 mL C6H14 and CH2Cl2/C6H14（1∶1） as elution.The recoveries of saturated and aromatics hydrocarbons are 98% and 99%，respectively.However, the co-eluting phenomenon exists between aromatic（such as naphthalene and its methyl homologues，acenaphthylene，etc.） and saturated hydrocarbons through the silica cartridges.The determination results of crude oil sample indicate that the elution behaviors of the saturated and aromatic hydrocarbon fractions with C6H14，CH2Cl2-C6H14（1∶1） are completely consisitent with the standard mixtures through the silica cyanopropyl cartridges.With the advantages of practicality，reliablity，easy to operate，low reagent consumption and good precision（ with RSDs of 4.2%-8.5% ），the method is suitable for the quantification of crude oil samples with complicated matrices.