A method for the simultaneous determination of four tocopherols in vegetable oils by reverse phase high performance liquid chromatography（RP-HPLC） was established.The sample was extracted with methanol under ultrasonic.The chromatographic separation was carried out on a C30 column（4.6 mm i.d.×250 mm，5 μm） using fluorescence detection at 290 nm of excitation wavelength and 340 nm of emission wavelength.Methanol-water-tert-butyl methyl ether（60∶15∶25）was used as mobile phase by isocratic elution.Results showed that good linear ranges for 4 tocopherols were obtained from 0.050 mg/L to 100.0 mg/L with correlation coefficients（r2）larger than 0.999.The mean recoveries were in the range of 81.3%-93.2% at three spiked levels of 0.50，50.0，500.0 mg/kg，and the relative standard deviations（RSDs） were less than 6%.The limits of detection（LODs） were 0.10 mg/kg for α-tocopherol，0.05 mg/kg for β- and γ-tocopherol，and 0.02 mg/kg for δ-tocopherol. The method is accurate，sensitive and reliable，and could be applied in the real sample analysis.