1. 福建农林大学食品科学学院
2. 福建出入境检验检疫局检验检疫技术中心
3. 福州大学化学系
4. 食品安全分析与检测教育部重点实验室
5. 福建省食品安全分析与检测重点实验室
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张盼盼, 钱疆, 杨方, 等. 高效液相色谱法检测食品中姜黄素类化合物[J]. 分析测试学报, 2013,32(9):1127-1130.
Determination of Curcuminoids in Food by High Performance Liquid Chromatography[J]. 2013,32(9):1127-1130.
建立了食品中姜黄素类化合物的液相色谱分析方法。采用甲醇提取试样中姜黄素及其同系物去甲氧基姜黄素和双去甲氧基姜黄素,以YMCTM Carotenoid色谱柱(250 mm×4.6 mm,5 μm)分离,甲醇水溶液(A)-甲基叔丁基醚(B)梯度洗脱。对所分析化合物的稳定性、前处理条件及色谱条件进行考察。3种分析物在0.5~100 μg/mL范围内线性关系良好,相关系数为0.997 7~0.999 8。在10~100 mg/kg 加标浓度范围内,平均回收率为72.2%~102%,相对标准偏差为3.4%~13.8%。对姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素的检出限分别为6、5、5 mg/kg。方法稳定、可靠,已应用于实际样品的检测。
An effective high performance liquid chromatographic(HPLC) method was developed for the determination of three curcuminoids,e.g.curcumin(CurⅠ),demethoxycurcumin(CurⅡ) and bisdemethoxycurcumin(Cur Ⅲ),in food.The analytes were extracted from homogenised samples using methanol,and separated on a YMCTM Carotenoid column(250 mm×4.6 mm,5 μm) by using methanol-water(A) and methyl teritary butyl ether(B) as mobile phase.The influences of experimental conditions were investigated.Under the optimal conditions,the calibration curves were linear in the range of 0.5-100 μg/mL,with correlation coefficients of 0.997 7-0.999 8.The average recoveries at spiked concentration range of 10-100 mg/kg ranged from 72.2% to 102% with relative standard deviations of 3.4%-13.8%.The limits of detection(LOD) of curcumin,demethoxycurcumin and bisdemethoxycurcumin were 6,5 mg/kg and 5 mg/kg,respectively.This method is suitable for the determination of curcuminoids in food.
姜黄素类化合物高效液相色谱分析
curcuminoidshigh-performance liquid chromatography(HPLC)determination
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