A method was developed for the determination of furazolidone（FZD），furaltadone（FTD），furacilin（NFZ）and nitrofurantoin（NFT） residues in feedstuff by isotope dillution high performance liquid chromatography-tandem mass spectrometry.After homogenization，samples were extracted with acetonitrile.After centrifugation，the extract was evaporated to dryness with nitrogen at 40℃，and redissolved with acetonitrile-0.05%ammonium acetate solution.The solution was defatted with hexane and cleaned up with neutral alumina solid phase extraction cartridge.The targeted compounds were seperated on a Sunfire C18（150 mm×2.1 mm，5 μm） column by using methanol-0.05% ammonium acetate as mobile phase，and detected with electrospray ionization（ESI） under positive and negative switching scan mode， in which negative scan mode was used for NFZ and NFT,respectively，and positive scan mode was used for FZD and FTD.The detection limits were 2.0 μg/kg for FZD and NFT and 3.0 μg/kg for FTD and NFZ,respectively.The quantitation limits were 5.0 μg/L for FZD and NFT，and 10.0 μg/kg for FTD and NFZ,respectively.The linear ranges of the target compounds ranged from 5.0 μg/L to 100.0 μg/L with correlation coefficients greater than 0.99.The recoveries of four compounds in spiked feed for tilapia were in the range of 78%-107% with relative standard deviations（RSDs） less than 12%.The method is rapid and accurate，and is suitable for the detection of four nitrofuran drugs in feedstuff.