An ion chromatographic method with direct conductivity detection for the determination of N-methyl，ethyl morpholinium cation（［MEMo］+），N-methyl，propyl morpholinium cation（［MPMo］+） two morpholinium ionic liquid cations was developed using a cation-exchange column and ethylenediamine-acetonitrile as mobile phase.The effects of column type，mobile phase and column temperature on retention and separation of the cations were investigated.The retention rules of the cations under different chromatographic conditions were formulated.The results indicated that the retention process of morpholinium cations retained in line with the ion-exchange mode，wihich was an exothermic process.The successful separation of the two morpholinium ionic liquid cations was achieved using the optimized mobile phase of 0.1 mmol/L ethylenediamine-0.5% acetonitrile（adjusted to pH 5.0 with hydrochloric acid） at a flow rate of 1.0 mL/min and a column temperature of 30 ℃.Under the optimal conditions，the two morpholinium ionic liquid cations could be successfully separated in 4 min.The detection limits（S/N=3） for ［MEMo］+ and ［MPMo］+ were 0.08 mg/L and 0.13 mg/L，respectively.The relative standard deviations（RSDs，n=5） of the chromatographic peak areas for the cations were no more than 1.5%.The method was successfully applied in the determination of morpholinium bromide ionic liquid samples synthesized by chemistry laboratory.The recoveries of morpholinium cations after spiked were in the range of 94.0%-106.0%.The method was proved to be accurate，reliable and rapid，and could meet the requirements for quantitative analysis of morpholinium ionic liquid samples.