New carbon paste electrodes（HRP-MWCNTs-CILE） were prepared by using multi-walled carbon nanotubes（MWCNTs） and room temperature ionic liquid 1-ethyl-3-methylimidazolium hexafluorophosphate（［EMIM］PF6） as binder.Then horseradish peroxidase（HRP） was modified on the surface of the electrode.The direct electrochemical characteristics of the modified electrodes were investigated by cyclic voltammetry（CV） and chronoamperometry（it）.A pair of approximate reversible redox peaks on CVs were observed in phosphate buffer.The enzyme electrodes showed good catalytic activity for hydrogen peroxide and good anti interference ability and stability.Under the optimal conditions,the biosensor showed a wide linear response to H2O2 in the range of 7.0×10-6-3.0×10-3 mol/L with a detection limit（S/N=3） of 2.5×10-6 mol/L.The sensor showed the characteristics of simple preparation,low cost,fast response and good application prospect.