A two-step microreactor was designed,and the influence of geometry parameters on reaction was optimized.To improve hybrid in the mix channel of the microreactor,the rib obstacles that can induce chaotic convection were designed.The ratio of height of obstacle and channel,the ratio of width of obstacle and spacing,and the width of obstacle were optimized with the software based on finite element theory.The serpentine channel was chose as the reaction unit of the microreactor,and the influences of the channel length,reaction time and initial concentration on reaction were investigated.Furthermore,a two-step microreactor for oxidation of glucose was designed.