A method was established for the simultaneous determination of seven sulfonylurea hypoglycemic agents（e.g.glipizide，tolbutamide，tolazamide，gliclazide，glibenclamide，glimepiride and glurenor) in health care products by solid phase extraction/high performance liquid chromatography（SPE/HPLC).Various samples were extracted with methanol by ultrasound，the extract was cleaned up and concentrated on an LC-C18 SPE column.The chromatographic separation was performed on an Agilent Zorbax SB-C18 column（250 mm×4.6 mm，5 μm) using acetonitrile（A)-0.02% phosphoric acid solution（B) as mobile phase by gradient elution at a flow rate of 1.0 mL/min，a column temperature of 30 ℃ and an ultraviolet（UV) detection wavelength of 228 nm.The seven sulfonylurea hypoglycemic agents showed good linear relationships in the range of 0.2-20 μg/mL with correlation coefficients larger than 0.999 9.The limits of detection（S/N≥3) were 0.1 mg/kg for seven target compounds.The spiked recoveries of seven target compounds in five different health care products ranged from 81% to 115% with relative standard deviations less than 9%.