A simple analysis method was developed for simultaneous determination of 1-propyl alcohol，diethylene glycol，propylene glycol，glycerol，triethylene glycol in tobacco by gas chromatography-mass spectrometry/selected ion mode（GC-MS/SIM）with 1，4-butanediol as internal standard.40 mesh powdered tobacco sample was ultrasonic extracted by methanol for 60 min directly.Under the optimal conditions，the calibration curves were linear in the range of 1-100 μg/mL，and the limits of detection（LODs） were in the range of 0.05-0.48 μg/mL.The spiked recoveries of analytes ranged from 90.2% to 103.7% with RSDs of 0.9%-3.0%.This method was suitable for the determination of 1-propyl alcohol，diethylene glycol，propylene glycol，glycerol，triethylene glycol in real tobacco samples with high sensitivity and convenient pre-treatment.