Simultaneous Determination of Para Red and Four Sudan Dyes Residues in Eggs by Dispersive Solid-phase Extraction Combined with High Performance Liquid Chromatography
Simultaneous Determination of Para Red and Four Sudan Dyes Residues in Eggs by Dispersive Solid-phase Extraction Combined with High Performance Liquid Chromatography[J]. 2013,32(11):1379-1383.
Simultaneous Determination of Para Red and Four Sudan Dyes Residues in Eggs by Dispersive Solid-phase Extraction Combined with High Performance Liquid Chromatography[J]. 2013,32(11):1379-1383.DOI:
建立了一种分散固相萃取结合高效液相色谱法测定鸡蛋样品中对位红及苏丹红Ⅰ~Ⅳ染料残留的分析方法。样品经正己烷超声提取,二醇基(Diol)硅胶吸附富集,乙腈洗脱后在Phenomenex Luna C18色谱柱(50 mm×2.0 mm,5 μm)上以乙腈-水为流动相梯度洗脱,在500 nm波长处检测,外标法定量。5种染料在0.1~10.0 mg?L-1范围具有良好的线性,相关系数均大于0.999,在低、中、高3个加标水平的平均回收率为81.2%~94.2%,相对标准偏差为3.4%~5.3%,检出限为0.018~0.030 mg?kg-1,定量下限为0.06~0.10 mg?kg-1。建立的方法准确快速,可用于鸡蛋中对位红和苏丹红类染料残留的快速检测。
Abstract
A method was established for the simultaneous determination of para red,sudan Ⅰ,sudanⅡ,sudan Ⅲ,and sudan Ⅳ residues in eggs by dispersive solid phase extraction(dSPE) combined with high performance liquid chromatography(HPLC).The samples were extracted with n-hexane by ultrasonic technique,and then enriched and cleaned up with Diol silica gel absorbent.The separation of the target compounds were performed on a Phenomenex Luna C18 column(50 mm×2.0 mm,5 μm) by using acetonitrile-water as mobile phase by gradient elution.The detection of the target compounds was performed with an ultraviolet-visible(UV-vis) detector at 500 nm and quantified by the external standard method.The results showed that the linear ranges of para red and four sudan dyes were between 0.1 mg?L-1and 10.0 mg?L-1 with correlation coefficients more than 0.999.The average recoveries at three spiked levels ranged from 81.2% to 94.2% with relative standard deviations(RSDs) of 3.4%-5.3%.The limits of detection(LODs) were in the range of 0.018-0.030 mg?kg-1,and the limits of quantitation(LOQs) were 0.06-0.10 mg?kg-1.The developed method is simple,rapid and sensitive,and is suitable for the determination of trace dyes residues in eggs.