A gas chromatographic（GC） method was established for the determination of three kinds of mono-glycerates（e.g.glycerate monocaprylin，glyceryl monodecanoate and glycery monolaurate） in bread.The sample was extracted with dichloromethane-n hexane（1∶1）.The extract was concentrated，then extracted with acetonitrile saturated with n-hexane，and detected by GC with the external standard method.The calibration curves for three mono-glycerates were linear in the range of 20-1 000 mg/L with correlation coefficients more than 0.999.The limits of quantitation of 3 monoglycerides are 12.8-35.2 mg/kg.The spiked recoveries were in the range of 81.1%-95.7% with RSDs of 2.9%-8.8%.The method has the advantages of simple operation，high sensitivity，good reproducibility and selectivity，and could meet the requirements for trace analysis of three monoglyceride in bread.