A high performance liquid chromatography-tandem mass spectrometric method for the determination of erythromycin A and its metabolites including erythromycin A enolether and anhydroerythromycin A was developed.The sample was extracted with pH 7.0 phosphate buffer and cleaned-up with HLB column.The detection of target compounds was performed by LC-MS with electrospray positive ionization under the multiple reaction monitoring(MRM) mode.The internal standard method was used for quantitative analysis.Under the optimal conditions,the calibration curves were linear in the range of 2.0-100.0 μg/L with correlation coefficients more than 0.996.The limits of detection and the limits of quantitation were 0.5 μg/kg and 2.0 μg/kg,respectively.The recoveries of three compounds at fortified levels of 2.0,5.0 and 10.0 μg/kg ranged from 70.5% to 109.2% with RSDs less than 10%.The method,with higher recoveries and good precision,was suitable for the determination of erythromycin A and its metabolites in honey.