Biogenic monoamine is known as an important series of neurotransmitters.They are involved in a variety of biological activities，such as mental activity，stress and emotion controlling，learning，physical movement，etc.The determination of monoamine neurotransmitters and their metabolites is significant for the diagnosis and control of many diseases，drug development and the neurological fundamental research.However，trace-level of basal monoamine and complex matrix of biological sample make their efficient separation and accuracy quantification extremely difficult.In this paper，the methods for the sample preparation of monoamine neurotransmitters in biological sample were reviewed，and the new progress in analysis of monoamine neurotransmitters was introduced.The future trends of monoamine neurotransmitters analysis was also prospected.