A high performance liquid chromatographic（HPLC）method was established for the simultaneous separation and determination of α-bromocinnamaldehyde（α-BCA），thiabendazole（TBI），triclosan（TCC） and triclocarban（TCS） in textiles.The samples were dissolved with ethyl acetate and extracted by ultrasonic extraction.The extract was concentrated and filtrated，then the separation of analytes was preformed on a symmetry C18（4.6 mm i.d.×250 mm，5 μm） column，with methanol-tetrahydrofuran-20 mmol/L NH4Ac as mobile phase（60∶20∶20） using isocratic elution at a flow rate of 0.8 mL/min.The photodiode array detector was set at 230，260，300 nm.The results indicated that four substances were completely separated in 15 min.The calibration curves showed good linearities in the ranges of 0.2-100 mg/L for α-BCA and TBI，0.1-100 mg/L for TCC，and 0.3-100 mg/L for TCS，with correlation coefficients of 0.998 8-0.999 9.The recoveries of four substances were in the range of 80.5%-104.9%，with RSDs below 7.2%.The quantitation limits for α-BCA，TBI，TCC，TCS（LOQ，S/N≥10） in three kinds of textiles were in the ranges of 0.2-0.5,0.2-0.4,0.1-0.2,0.3-0.6 mg/kg，respectively.This method was simple，rapid and sensitive，and was applied in the determination of organic antibacterial and antifungal agents in textiles with satisfactory results.