A new multi-residue analytical method was developed for the determination of 27 carbamate pesticides and their metabolites residues in ginger by liquid chromatography-tandem mass spectrometry(LC-MS/MS).The samples were homogeneously extracted with acetonitrile,desalted with NaCl,and then cleaned with NH2 solid-phase extraction column.The extracts were redissolved in a mixture of acetonitrile-water(1∶1),separated on an Agilent Eclipse Plus C18 column and analyzed by LC-MS/MS under multiple reaction monitoring mode via positive electrospray ionization.The quantitation was carried out by the matrix-matched external standard calibrations.The method showed excellent linearities(r2＞099) for all compounds in a certain concentration range.The limits of detection and the limits of quantitation were in the ranges of 0.05-2.0 μg/kg and 0.2-5.0 μg/kg,respectively.The recoveries at three spiked levels of 10,30,100 μg/kg ranged from 70.9% to 119.1% with relative standard deviations of 1.0%-10.8%.The method showed the advantages of simplicity,rapidness and sensitivity,and could meet the requirements for the determination of carbamates and their metabolites residues in ginger.