A solid phase extraction combined with ultra performance liquid chromatography-tandem mass spectrometric（SPE/UPLC-MS/MS） method was developed for the simultaneous determination of 12 synthetic colorants，including Acid Violet 49，Pigment Red 57，Pigment Red 53∶1，Acid Yellow 36，Rhodamine B，Basic Violet 3，Disperse Yellow 3，Pigment Orange 5，Sudan Ⅰ，Sudan Ⅱ,Sudan Ⅳ，Solvent Blue 35，in lip gloss，eye shadow，lipstick and other cosmetics.The cosmetics were extracted sequentially by ultrasonic technique with tetrahydrofuran（THF），dimethyl sulfoxide（DMSO） and methanol，cleaned up with HLB solid phase extraction column.The separation was performed on an Acquity UPLC BEH-C18（2.1 mm×50 mm，1.7 μm） column by gradient elution using acetonitrile-0.05% formic acid as mobile phase，then the isolates were analyzed under multiple reaction monitoring（MRM） mode，and quantified by the matrix matched external standard method.The results showed that the 12 investigated colorants displayed good peak shapes within 6 min.The calibration curves of 12 colorants were linear in the range of 0.5-800 μg?L-1，with correlation coefficients more than 0.99.The limits of quantitation（LOQ，S/N>10） were in the range of 1.4-2 000 μg?kg-1.The recoveries of 12 colorants in spiked lip gloss，eye shadow and lipstick samples ranged from 60.8% to 118.0% with relative standard deviations（RSD） of 2.3%-10.8%.The method was rapid，simple and sensitive，and was suitable for the determination and confirmation of the colorants in oil cosmetics，cream cosmetics and powder cosmetics.