A high performance liquid chromatographic（HPLC） methord was developed for the simultaneous determination of 14 forbidden colorants（Metanil Yellow,Solvent Blue 35,Solvent Red 49,Acid Violet 49,Tetramethylpararosaniline Chloride（TMPC）,Pigment Orange 5,Pigment Red 53,Pentamethyltriphenylmethane（PMTPE）,Sudan Red Ⅱ,Hexamethylenepararosaniline Chloride（HMPC）,Solvent Red 24,Rhodamine B,Disperse Yellow 3,Sudan Red Ⅰ） in cosmetics.Cosmetic samples were extracted and dispersed with tetrahydrofuran,and some matrixes were precipitated out after additon of ammonium acetate solution and methanol.After centrifuged and filtered，the extract was separated on an Agilent poroshell 120 EC-C18 column（2.7 μm，3.0 mm×100 mm） by a gradient elution with 10 mmol/L ammonium acetate-acetonitrile as mobile phase.Detection wavelengths were set at 416,514,590 nm,respectively.The qualitative analysis was based on chromatographic retention time and UV spectrum,and the quantitative analysis was performed by the standard curve method of peak area.The linear ranges of 14 analytes were in the range of 0.2-20 μg/mL,with correlation coefficients more than 0.999.The limits of quantitation（LOQs） were in the range of 3-10 μg/g.The average recoveries at three spiked levels ranged from 91.3% to 110.9% with RSDs less than 10%.This method was accurate,simple,sensitive and reliable,and could be used for the analysis of 14 forbidden colorants in cosmetics.