A fast and easy method for the screening and quantification of 111 pesticides residues in wines by liquid chromatography-Quadrupole/Orbitrap high-resolution mass spectrometry was developed.The wine samples were extracted with acetonitrile (containing 0.1% acetic acid),and salted out with anhydrous magnesium sulphate and anhydrous sodium acetate.After concentration,the analyte was dissolved with methanol and water,then cleaned up with primary secondary amine (PSA) to reduce matrix interference.Chromatographic analysis was carried out using a C18 column with acetonitrile-5 mmol/L ammonium acetate solution as mobile phase by gradient elution.111 pesticides residues were analyzed by high-resolution mass spectrometry in positive mode.Accurate mass extraction of the target pesticide compounds and data dependence threshold-triggered MS/MS minimized the matrices interference during the analysis.The linearities of all 111 pesticides ranged from 1 μg/L to 100 μg/L with correlation coefficients greater than 0.99.By detecting spiked samples,the limits of detection of the method were 5 μg/kg for all the pesticides residues and the recoveries were in the range of 63.3%-123.7% with RSDs of 3.2%-18.8%.This method could be used for high-throughput screening and confirmation of multiple pesticide residues in wines.