A hydrophilic interaction liquid chromatographic method was established for rapid screening of thiourea dioxide illegally added in food fillings.0.05% acetic acid was used for ultrasonic extraction of thiourea dioxide in ice water bath.The extraction solution was centrifuged,and the supernatant was tested after filtered through 0.45 μm cellulose micro pore membrane.The target compound was separated on a Venusil HILIC（4.6 mm×250 mm,5 μm） column at 25 ℃,using 0.02 mol/L ammonium acetate（glacial acetic acid adjust pH 4.5）–acetonitrile as mobile phase by gradient elution,and detected with photodiode array detector at 275 nm.The calibration curve showed a good linearity for thiourea dioxide within the range of 6.0-200 mg/L.The limits of detection（LOD）and limits of quantitation（LOQ） for the method were 22.5 mg/kg and 75.0 mg/kg,respectively.The spiked recoveries of thiourea dioxide in food fillings ranged from 92.7% to 107% with RSDs of 0.53%-3.8%.This method was rapid,accurate and repeatable,and was suitable for rapid screening thiourea dioxide in food fillings.