A dispersive solid phase extraction（d-SPE）/high-resolution benchtop Q Exactive mass spectrometric（HRMS） method was established for the rapid screening of pesticide multi-residue in green pepper.The samples were extracted with acetonitrile，and cleaned up by dispersive solid phase extraction with N-propyl ethlene diamine（PSA） and C18.The chromatographic analysis was performed on a BEH C18 column using acetonitrile（containing 0.1% formic acid） and 4 mmol/L ammonium formate（containing 0.1% formic acid） solution as mobile phases by a gradient elution program.The multi-residue pesticides were analyzed by Q Exactive high-resolution mass spectrometry in switching positive and negative modes,which were applied in rapid screening of pesticides for EU checking pepper samples.16 pesticide analytes were screened qualitatively from the checking pepper sample and were carried on the quantitative analysis.The method showed a good linearity in a certain concentration range for 16 pesticides with average recoveries of 81.6%-116.5% and RSDs of 0.4%-7.5%.The limits of quantitation（LOQs） for 16 pesticides in pepper were between 0.4 μg/kg and 2.0 μg/kg.The method was simple，sensitive and accurate，and was suitable for the rapid screening of multi-residue pesticides in agricultural products.